QuantumKitten2(Wednesday 5th of December 2007 05:23:35 AM)
Hmm... is the RISC holoenzyme the same for endogenous miRNAs and transfected siRNAs/shRNAs?
easternblot(Thursday 17th of January 2008 11:35:17 AM)
Yes, it's the same. The difference is earlier: miRNAs are first processed in the nucleus by Drosha - siRNA and shRNA (usually) are not. shRNA are ceaved by Dicer in the cytoplasm. siRNA resembles the product of Dicer. All three (miRNA processed by Drosha/Dicer, shRNA cleaved by Dicer, and siRNA) are about the same shape and size at this point and are all recognized by the RISC complex.
